Integron frequency of Escherichia coli strains from patients with urinary tract infection in Southwest of Iran
Miaad K Alkhudhairy1, Morteza Saki2, Sakineh Seyed–Mohammadi2, Nabi Jomehzadeh3, Saeed Khoshnood4, Mina Moradzadeh5, Sajjad Yazdansetad6
1 Community Health Department, College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, Kufa, Iraq
2 Department of Microbiology, Faculty of Medicine; Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Abadan School of Medical Sciences, Abadan, Iran
4 Student Research Committee, School of Medicine, Bam University of Medical Sciences, Bam, Iran
5 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
6 Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Department of Microbiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz; Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Source of Support: None, Conflict of Interest: None
Objective: To investigate the frequency of integrase genes intI1, intI2 and intI3 of Escherichia coli strains, and their association with resistance to routinely used antibiotics.
Methods: A total of 120 Escherichia coli strains were collected from patients with urinary tract infection in Ahvaz, Southwest of Iran. Antibiotic susceptibility testing was performed. The presence of intI1, intI2, and intI3 genes was determined by polymerase chain reaction.
Results: Antibiotic susceptibility testing disclosed the highest resistance rate to ampicillin (91.7%) followed by trimethoprim/sulfamethoxazole (65.8%), and ceftazidime (56.7%). The imipenem susceptibility rate was 91.7%. IntIl and intI2 were identified in 74 (61.6%) and 8 (6.6%) of Escherichia coli strains, respectively, but intI3 was not found in any isolates. The presence of integrons was significantly associated with resistance to ampicillin, trimethoprim/ sulfamethoxazole, ceftazidime, and ciprofloxacin antibiotics (P<0.05).
Conclusions: The high resistant Escherichia coli isolates harboring class 1 integrons (intI1) were detected in patients with urinary tract infection in our region. Therefore, preventive strategies are necessary to restrict further dissemination of resistant strains.