Journal of Acute Disease

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 10  |  Issue : 3  |  Page : 117--121

Detection of oqxA and oqxB efflux pump genes among nosocomial coliform bacilli: An observational cross-sectional study


Basant Mostafa Gabr1, Afaf Sayed Ahmed Zamzam1, Eman Ahmed Eisa1, Ghada Foad El-Baradey2, Maii Atef Shams Eldeen1 
1 Microbiology and Immunology Department, Faculty of Medicine, Tanta University, El-Gaish, Tanta Qism 2, Tanta, Gharbia, Egypt
2 Anesthesiology and Intensive Care Department, Faculty of Medicine, Tanta University, El-Gaish, Tanta Qism 2, Tanta, Gharbia, Egypt

Correspondence Address:
Basant Mostafa Gabr
Microbiology and Immunology Department, Faculty of Medicine, Tanta University, El-Gaish, Tanta Qism 2, Tanta, Gharbia
Egypt

Objectives: To identify and test the antibiotic susceptibility of nosocomial coliform bacilli and investigate the presence of oqxA and oqxB genes among the multidrug-resistant (MDR) phenotypes. Methods: One hundred and twenty different healthcare-associated infection samples were collected. Coliform bacilli were isolated, identified by conventional methods, and then antibiotic susceptibility tests were done using the VITEK2 system and disk diffusion methods. OqxAB operon was identified using a conventional PCR-based technique. oqxA and oqxB genes were compared between MDR Klebsiella pneumonia (K. pneumonia) phenotypes and MDR Escherichia coli (E. coli) phenotypes. Besides, oqxAB operons were compared between phenotypes of K. pneumonia and E. coli isolates. Results: Seventy coliform bacilli were isolated with the predominance of K. pneumonia and E. coli. Besides, 82.1% of K. pneumonia strains and 53.3% of E. coli isolates were MDR phenotypes. Significant more oqxB genes alone were found in MDR E. coli than that in MDR K. pneumoniae phenotypes (χ2=10.160, P=0.003). OqxAB operon was significantly more in MDR phenotypes of E. coli than that in the susceptible phenotypes (P<0.001). There was significantly less of this operon in susceptible E. coli isolates than that in susceptible K. pneumoniae isolates (P<0.001). OqxAB positive isolates that were also resistant to fluoroquinolones, tetracycline, trimethoprim, and chloramphenicol, most probably suggested functional pumps. Conclusions: MDR coliform bacilli are strongly implicated in healthcare-associated infection. Attention should be paid to the presence of oqxAB pump, as an important mechanism in the development of resistance against many antimicrobials because it contributes to co-resistance with other categories; therefore, this pump could be a good target for efflux pump inhibitors.


How to cite this article:
Gabr BM, Zamzam AS, Eisa EA, El-Baradey GF, Eldeen MA. Detection of oqxA and oqxB efflux pump genes among nosocomial coliform bacilli: An observational cross-sectional study.J Acute Dis 2021;10:117-121


How to cite this URL:
Gabr BM, Zamzam AS, Eisa EA, El-Baradey GF, Eldeen MA. Detection of oqxA and oqxB efflux pump genes among nosocomial coliform bacilli: An observational cross-sectional study. J Acute Dis [serial online] 2021 [cited 2021 Jul 30 ];10:117-121
Available from: http://www.jadweb.org/article.asp?issn=2221-6189;year=2021;volume=10;issue=3;spage=117;epage=121;aulast=Gabr;type=0